• Product nameAnti-USP28 antibody
    See all USP28 primary antibodies
  • Description
    Rabbit polyclonal to USP28
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    A region between residue 500 and 550 of human USP28 (NP_065937.1).

  • Positive control
    • Whole cell lysate from HeLa cells.


Associated products


Our Abpromise guarantee covers the use of ab70894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionDeubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
    • Sequence similaritiesBelongs to the peptidase C19 family. USP28 subfamily.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Post-translational
      Degradaded upon nickel ion level or hypoxia exposure.
      Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
    • Cellular localizationNucleus > nucleoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 28 antibody
      • KIAA1515 antibody
      • Ubiquitin carboxyl terminal hydrolase 28 antibody
      • Ubiquitin carboxyl-terminal hydrolase 28 antibody
      • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
      • Ubiquitin specific peptidase 28 antibody
      • Ubiquitin specific processing protease 28 antibody
      • Ubiquitin specific protease 28 antibody
      • Ubiquitin thioesterase 28 antibody
      • Ubiquitin thiolesterase 28 antibody
      • Ubiquitin-specific-processing protease 28 antibody
      • UBP28_HUMAN antibody
      • USP 28 antibody
      • USP28 antibody
      • USP28 protein antibody
      see all

    Anti-USP28 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP28 with ab70894 at 1/5000 (0.2µg/ml). Detection: DAB.
    • Detection of USP28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70893 (Lane 1) and ab70894 (Lane 2) at 3 µg/mg lysate for IP. ab70893 was used at 1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.

    References for Anti-USP28 antibody (ab70894)

    ab70894 has not yet been referenced specifically in any publications.

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