Anti-USP28 antibody [EPR4250(2)] (ab110744)

Overview

  • Product nameAnti-USP28 antibody [EPR4250(2)]
    See all USP28 primary antibodies
  • Description
    Rabbit monoclonal [EPR4250(2)] to USP28
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human USP28.

  • Positive control
    • 293T, SW480, HeLa, and A431 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionDeubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
    • Sequence similaritiesBelongs to the peptidase C19 family. USP28 subfamily.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Post-translational
      modifications
      Degradaded upon nickel ion level or hypoxia exposure.
      Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
    • Cellular localizationNucleus > nucleoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 28 antibody
      • KIAA1515 antibody
      • Ubiquitin carboxyl terminal hydrolase 28 antibody
      • Ubiquitin carboxyl-terminal hydrolase 28 antibody
      • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
      • Ubiquitin specific peptidase 28 antibody
      • Ubiquitin specific processing protease 28 antibody
      • Ubiquitin specific protease 28 antibody
      • Ubiquitin thioesterase 28 antibody
      • Ubiquitin thiolesterase 28 antibody
      • Ubiquitin-specific-processing protease 28 antibody
      • UBP28_HUMAN antibody
      • USP 28 antibody
      • USP28 antibody
      • USP28 protein antibody
      see all

    Anti-USP28 antibody [EPR4250(2)] images



    • Predicted band size : 122 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab110744 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab110744 was shown to specifically react with USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE.  Ab110744 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-USP28 antibody [EPR4250(2)] (ab110744) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 122 kDa

    References for Anti-USP28 antibody [EPR4250(2)] (ab110744)

    ab110744 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Xenopus laevis Cell lysate - whole cell (egg extracts)
    Specification egg extracts
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Submitted Sep 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"