Anti-USP3 antibody (ab101473)

Overview

  • Product nameAnti-USP3 antibody
    See all USP3 primary antibodies
  • Description
    Rabbit polyclonal to USP3
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chicken, Dog, Pig, Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human USP3.

  • Positive control
    • This antibody gave a positive signal in the following cell lysates: U20S; HeLa Nuclear; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab101473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. Use with paraformaldehyde-fixed cells, permeabilized with 0.5% Triton X100-PBS.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

Target

  • FunctionHydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
  • Tissue specificityExpressed in all tissues examined, with strongest expression in pancreas.
  • Sequence similaritiesBelongs to the peptidase C19 family. USP3 subfamily.
    Contains 1 UBP-type zinc finger.
  • DomainBoth protease activity and an intact zinc finger are required for H2A monodeubiquitination.
  • Cellular localizationNucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 3 antibody
    • EC 3.1.2.15 antibody
    • SIH003 antibody
    • Ubiquitin carboxyl-terminal hydrolase 3 antibody
    • Ubiquitin specific peptidase 3 antibody
    • Ubiquitin specific processing protease 3 antibody
    • Ubiquitin Specific Protease 3 antibody
    • Ubiquitin thioesterase 3 antibody
    • Ubiquitin thiolesterase 3 antibody
    • Ubiquitin-specific-processing protease 3 antibody
    • UBP antibody
    • UBP3_HUMAN antibody
    • Usp3 antibody
    see all

Anti-USP3 antibody images

  • All lanes : Anti-USP3 antibody (ab101473) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 18 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ab101473 (1/500) staining USP3 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References for Anti-USP3 antibody (ab101473)

ab101473 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Egg extracts)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Egg extracts
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 20 2013

Thank you for your reply.

Either of these antibodies should work well in your assay. Ab101473 has already been tested with HeLa lysates and works well, however I know that the exposure time was an important variable in your decision. Since th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"