Anti-USP3 antibody (ab101473)
Key features and details
- Rabbit polyclonal to USP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-USP3 antibody
See all USP3 primary antibodies -
Description
Rabbit polyclonal to USP3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chicken, Dog, Pig, Macaque monkey -
Immunogen
Synthetic peptide corresponding to Human USP3 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab115142) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
Target
-
Function
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. -
Tissue specificity
Expressed in all tissues examined, with strongest expression in pancreas. -
Sequence similarities
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger. -
Domain
Both protease activity and an intact zinc finger are required for H2A monodeubiquitination. -
Cellular localization
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin. - Information by UniProt
-
Database links
- Entrez Gene: 9960 Human
- GenBank: NM_006537 Human
- Omim: 604728 Human
- SwissProt: Q9Y6I4 Human
- Unigene: 458499 Human
-
Alternative names
- Deubiquitinating enzyme 3 antibody
- EC 3.1.2.15 antibody
- SIH003 antibody
see all
Images
-
All lanes : Anti-USP3 antibody (ab101473) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab101473 has been referenced in 4 publications.
- Rhie BH et al. Ubiquitin-Specific Protease 3 Deubiquitinates and Stabilizes Oct4 Protein in Human Embryonic Stem Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34070420
- Zhou Q et al. Ubiquitin-specific protease 3 targets TRAF6 for deubiquitination and suppresses IL-1ß induced chondrocyte apoptosis. Biochem Biophys Res Commun 514:482-489 (2019). PubMed: 31056254
- Wu Y et al. USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5. J Biol Chem 294:17837-17847 (2019). PubMed: 31624151
- Wang Z et al. Downregulated USP3 mRNA functions as a competitive endogenous RNA of SMAD4 by sponging miR-224 and promotes metastasis in colorectal cancer. Sci Rep 7:4281 (2017). WB ; Human . PubMed: 28655924