Anti-USP3 antibody (ab80597)
Key features and details
- Rabbit polyclonal to USP3
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP3 antibody
See all USP3 primary antibodies -
Description
Rabbit polyclonal to USP3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide (Human) mapped to a region between residues 471 and 521
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Positive control
- IP: HeLa whole cell lysate; IHC: human ovarian carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.5% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. -
Tissue specificity
Expressed in all tissues examined, with strongest expression in pancreas. -
Sequence similarities
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger. -
Domain
Both protease activity and an intact zinc finger are required for H2A monodeubiquitination. -
Cellular localization
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin. - Information by UniProt
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Database links
- Entrez Gene: 9960 Human
- GenBank: NM_006537 Human
- Omim: 604728 Human
- SwissProt: Q9Y6I4 Human
- Unigene: 458499 Human
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Alternative names
- Deubiquitinating enzyme 3 antibody
- EC 3.1.2.15 antibody
- SIH003 antibody
see all
Images
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USP3 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab80597 at 3 µg per mg lysate. Western blot was performed on the immunoprecipitate using ab80597 at 1.0 µg/mL.
Lane 1: ab80597 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP3 with ab80597 at 1/1000 (1µg/ml). Detection: DAB.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab80597 has been referenced in 1 publication.
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835