Overview

  • Product name
  • Description
    Rabbit polyclonal to USP3
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) mapped to a region between residues 471 and 521

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use a concentration of 2 - 5 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
    • Tissue specificity
      Expressed in all tissues examined, with strongest expression in pancreas.
    • Sequence similarities
      Belongs to the peptidase C19 family. USP3 subfamily.
      Contains 1 UBP-type zinc finger.
    • Domain
      Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.
    • Cellular localization
      Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 3 antibody
      • EC 3.1.2.15 antibody
      • SIH003 antibody
      • Ubiquitin carboxyl-terminal hydrolase 3 antibody
      • Ubiquitin specific peptidase 3 antibody
      • Ubiquitin specific processing protease 3 antibody
      • Ubiquitin Specific Protease 3 antibody
      • Ubiquitin thioesterase 3 antibody
      • Ubiquitin thiolesterase 3 antibody
      • Ubiquitin-specific-processing protease 3 antibody
      • UBP antibody
      • UBP3_HUMAN antibody
      • Usp3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP3 with ab80597 at 1/1000 (1µg/ml). Detection: DAB.

    References

    ab80597 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab80597.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up