The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 2 - 5 µg/ml.
Application notesIs unsuitable for WB.
FunctionHydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Tissue specificityExpressed in all tissues examined, with strongest expression in pancreas.
Sequence similaritiesBelongs to the peptidase C19 family. USP3 subfamily. Contains 1 UBP-type zinc finger.
DomainBoth protease activity and an intact zinc finger are required for H2A monodeubiquitination.
Cellular localizationNucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.