The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Tissue specificityExpressed in all tissues examined, with strongest expression in pancreas.
Sequence similaritiesBelongs to the peptidase C19 family. USP3 subfamily. Contains 1 UBP-type zinc finger.
DomainBoth protease activity and an intact zinc finger are required for H2A monodeubiquitination.
Cellular localizationNucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
ab84702, at 1/100 dilution, staining USP3 in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry using a Red fluorescent goat anti-rabbit IgG highly cross adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.