Overview

  • Product nameAnti-USP30 antibody
    See all USP30 primary antibodies
  • Description
    Rabbit polyclonal to USP30
  • Specificityab74368 antibody detects endogenous levels of total USP30 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human USP30.

  • Positive control
    • HeLa cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab74368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/10000.

Target

  • FunctionMay participate in the maintenance of mitochondrial morphology.
  • Tissue specificityExpressed in skeletal muscle, pancreas, liver and kidney.
  • Sequence similaritiesBelongs to the peptidase C19 family.
  • Cellular localizationMitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 30 antibody
    • FLJ40511 antibody
    • Ub-specific protease 30 antibody
    • Ubiquitin carboxyl terminal hydrolase 30 antibody
    • Ubiquitin carboxyl-terminal hydrolase 30 antibody
    • Ubiquitin specific peptidase 30 antibody
    • Ubiquitin specific processing protease 30 antibody
    • Ubiquitin thioesterase 30 antibody
    • Ubiquitin thiolesterase 30 antibody
    • Ubiquitin-specific-processing protease 30 antibody
    • UBP30_HUMAN antibody
    • usp30 antibody
    see all

Anti-USP30 antibody images

  • All lanes : Anti-USP30 antibody (ab74368) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 26 kDa,33 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab74368 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74368, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-USP30 antibody (ab74368)

ab74368 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"