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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab74368 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/500 - 1/1000. Predicted molecular weight: 59 kDa.
May participate in the maintenance of mitochondrial morphology.
Expressed in skeletal muscle, pancreas, liver and kidney.
Belongs to the peptidase C19 family.
Mitochondrion outer membrane.
Information by UniProt
Deubiquitinating enzyme 30 antibody
Ub-specific protease 30 antibody
Anti-USP30 antibody images
Western blot - USP30 antibody (ab74368)
All lanes : Anti-USP30 antibody (ab74368) at 1/500 dilution Lane 1 : HeLa cell extract Lane 2 : HeLa cell extract with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 59 kDa Observed band size : 59 kDa Additional bands at : 26 kDa,33 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-USP30 antibody(ab74368)
ICC/IF image of ab74368 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74368, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-USP30 antibody (ab74368)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"