Overview

  • Product name
  • Description
    Rabbit polyclonal to USP32
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 200 and 250 of human USP32 (NP_115971.2).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 182 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities
      Belongs to the peptidase C19 family.
      Contains 1 DUSP domain.
      Contains 3 EF-hand domains.
    • Cellular localization
      Membrane. Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 32 antibody
      • NY REN 60 antibody
      • NYREN60 antibody
      • Renal carcinoma antigen NY REN 60 antibody
      • Renal carcinoma antigen NY-REN-60 antibody
      • Ubiquitin carboxyl-terminal hydrolase 32 antibody
      • Ubiquitin specific peptidase 32 antibody
      • Ubiquitin specific processing protease 32 antibody
      • Ubiquitin specific protease 32 antibody
      • Ubiquitin thioesterase 32 antibody
      • Ubiquitin thiolesterase 32 antibody
      • Ubiquitin-specific-processing protease 32 antibody
      • UBP32_HUMAN antibody
      • USP10 antibody
      • USP32 antibody
      see all

    Images

    • All lanes : Anti-USP32 antibody (ab86792) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 182 kDa
      Observed band size : 182 kDa
      Additional bands at : 150 kDa,60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of USP32 by Western Blot of Immunprecipitate.
      ab86792, at 1 µg/ml, staining USP32 in HeLa whole cell lysate immunoprecipitated using ab86792 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 minutes.

    References

    ab86792 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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