Overview

  • Product nameAnti-USP33 antibody
    See all USP33 primary antibodies
  • Description
    Rabbit polyclonal to USP33
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide within Human USP33 aa 425-475. The exact sequence is proprietary. (GeneID 23032)
    Database link: NP_055832.3

Properties

Applications

Our Abpromise guarantee covers the use of ab71715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionDeubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the peptidase C19 family. USP20/USP33 subfamily.
      Contains 2 DUSP domains.
      Contains 1 UBP-type zinc finger.
    • DomainThe UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue.
    • Post-translational
      modifications
      Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
    • Cellular localizationCytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 33 antibody
      • EC 3 1 2 15 antibody
      • hVDU1 antibody
      • KIAA1097 antibody
      • MGC16868 antibody
      • OTTHUMP00000011234 antibody
      • OTTHUMP00000011330 antibody
      • OTTHUMP00000011331 antibody
      • pVHL interacting deubiquitinating enzyme 1 antibody
      • Ubiquitin carboxyl terminal hydrolase 33 antibody
      • Ubiquitin carboxyl-terminal hydrolase 33 antibody
      • Ubiquitin specific peptidase 33 antibody
      • Ubiquitin specific processing protease 33 antibody
      • ubiquitin specific protease 33 antibody
      • Ubiquitin thioesterase 33 antibody
      • Ubiquitin thiolesterase 33 antibody
      • Ubiquitin-specific-processing protease 33 antibody
      • UBP33_HUMAN antibody
      • USP 33 antibody
      • Usp33 antibody
      • VDU 1 antibody
      • VDU1 antibody
      • VHL interacting deubiquitinating enzyme 1 antibody
      • VHL-interacting deubiquitinating enzyme 1 antibody
      see all

    Anti-USP33 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling USP33 with ab71715 at 1/1000 (1µg/ml). Detection: DAB.

    References for Anti-USP33 antibody (ab71715)

    ab71715 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"