The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 387 kDa.
Use a concentration of 10 µg/ml.
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (ie. Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently).
Use at an assay dependent concentration.
FunctionUbiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins.
Tissue specificityExpressed in brain at low level.
Sequence similaritiesBelongs to the peptidase C19 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.