Overview

  • Product nameAnti-USP34 antibody
    See all USP34 primary antibodies
  • Description
    Mouse monoclonal to USP34
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog
  • Immunogen

    Recombinant fragment of Human USP34 with tag: LALLLSVHTP KQLNPALIPT LQELLSKCRT CLQQRNSLQE QEAKERKTKD DEGATPIKRR RVSSDEEHTV DSCISDMKTE TREVLTPTST SDNETRDSSI I (NP_055524, 3296 a.a. ~ 3396 a.a).

  • Positive control
    • HeLa cells. A549 cell lysate. Human seminoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab68343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 387 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (ie. Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently).
ELISA Use at an assay dependent concentration.

Target

  • FunctionUbiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins.
  • Tissue specificityExpressed in brain at low level.
  • Sequence similaritiesBelongs to the peptidase C19 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 34 antibody
    • FLJ43910 antibody
    • KIAA0570 antibody
    • KIAA0729 antibody
    • MGC104459 antibody
    • Ubiquitin carboxyl terminal hydrolase 34 antibody
    • Ubiquitin carboxyl-terminal hydrolase 34 antibody
    • Ubiquitin specific processing protease 34 antibody
    • Ubiquitin specific protease 34 antibody
    • Ubiquitin thioesterase 34 antibody
    • Ubiquitin thiolesterase 34 antibody
    • Ubiquitin-specific-processing protease 34 antibody
    • UBP34_HUMAN antibody
    • USP 34 antibody
    • Usp34 antibody
    see all

Anti-USP34 antibody images

  • Immunoperoxidase of monoclonal antibody to USP34 ab68343 on formalin-fixed paraffin-embedded human seminoma. Antibody concentration 3ug/ml.
  • Immunofluorescence of monoclonal antibody to USP34 ab68343 on HeLa cells. Antibody concentration 10 ug/ml.
  • Detection limit for recombinant tagged USP34 is approximately 1ng/ml as a capture antibody.
  • Anti-USP34 antibody (ab68343) at 1 µg/ml + immunogen at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 387 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

References for Anti-USP34 antibody (ab68343)

ab68343 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Human Cell (U2OS)
Specification U2OS
Permeabilization Yes - Methanol
Fixative Paraformaldehyde
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Submitted Mar 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"