Overview

  • Product nameAnti-USP37 antibody
    See all USP37 primary antibodies
  • Description
    Rabbit polyclonal to USP37
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 930-979 of Human USP37 (NP_065986.1)

  • Positive control
    • HeLa whole cell lysate 239T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 110 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Tissue specificityExpressed in brain and prostate.
  • Sequence similaritiesBelongs to the peptidase C19 family.
    Contains 3 UIM (ubiquitin-interacting motif) repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 3 antibody
    • Deubiquitinating enzyme 37 antibody
    • EC 3.1.2.15 antibody
    • KIAA1594 antibody
    • MGC117261 antibody
    • OTTHUMP00000164146 antibody
    • tmp_locus_50 antibody
    • Ubiquitin carboxyl-terminal hydrolase 37 antibody
    • Ubiquitin specific peptidase 37 antibody
    • Ubiquitin specific processing protease 37 antibody
    • Ubiquitin specific protease 37 antibody
    • Ubiquitin thioesterase 37 antibody
    • Ubiquitin thiolesterase 37 antibody
    • Ubiquitin-specific-processing protease 37 antibody
    • UBP37_HUMAN antibody
    • USP37 antibody
    see all

Anti-USP37 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling USP37 with ab72199 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-USP37 antibody (ab72199) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 110 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95-117 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab72199 at 3µg/mg lysate. 20% of the immunoprecipitate was used for Western blotting and detected using ab72199 at 1µg/ml. Bands were exposed by chemiluminescence with an exposure time of 10 seconds.

References for Anti-USP37 antibody (ab72199)

ab72199 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine)
Sample Human Cell lysate - whole cell (Osteoblast)
Specification Osteoblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Carlos le Sage

Verified customer

Submitted Aug 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"