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Rabbit polyclonal to USP37
Predicted to work with:
Synthetic peptide, corresponding to C terminal amino acids 930-979 of Human USP37 (NP_065986.1)
HeLa whole cell lysate
239T whole cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 110 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Expressed in brain and prostate.
Belongs to the peptidase C19 family.
Contains 3 UIM (ubiquitin-interacting motif) repeats.
Information by UniProt
Deubiquitinating enzyme 3 antibody
Deubiquitinating enzyme 37 antibody
EC 126.96.36.199 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP37 antibody (ab72199)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling USP37 with ab72199 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-USP37 antibody (ab72199)
All lanes :
Anti-USP37 antibody (ab72199) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
120 kDa (
why is the actual band size different from the predicted?
Additional bands at:
95-117 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-USP37 antibody (ab72199)
Immunoprecipitation of HeLa whole cell lysate (1mg) using ab72199 at 3µg/mg lysate. 20% of the immunoprecipitate was used for Western blotting and detected using ab72199 at 1µg/ml. Bands were exposed by chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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