Overview

  • Product nameAnti-USP38 antibody
    See all USP38 primary antibodies
  • Description
    Rabbit polyclonal to USP38
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Pig, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 700 and 750 of human USP38 (NP_115946.2)

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2,000 - 1/10,000. Detects bands of approximately 31, 68, and 117 kDa (predicted molecular weight: 117 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityHighly expressed in skeletal muscle. Expressed in adrenal gland.
    • Sequence similaritiesBelongs to the peptidase C19 family.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1891 antibody
      • Deubiquitinating enzyme 38 antibody
      • HP43.8KD antibody
      • Ubiquitin carboxyl terminal hydrolase 38 antibody
      • Ubiquitin carboxyl-terminal hydrolase 38 antibody
      • Ubiquitin specific peptidase 38 antibody
      • Ubiquitin specific processing protease 38 antibody
      • Ubiquitin thioesterase 38 antibody
      • Ubiquitin thiolesterase 38 antibody
      • Ubiquitin-specific-processing protease 38 antibody
      • UBP38_HUMAN antibody
      • USP 38 antibody
      • USP38 antibody
      see all

    Anti-USP38 antibody images

    • All lanes : Anti-USP38 antibody (ab72244) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 117 kDa
      Observed band size : 117 kDa
      Additional bands at : 31 kDa,68 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of USP38.
      Lane 1: ab72244 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72244 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Detection:Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-USP38 antibody (ab72244)

    ab72244 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (MCF7 breast cancer cells)
    Loading amount 20 µg
    Specification MCF7 breast cancer cells
    Treatment cells transfected with GFP or GFP-USP38 vector
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 09 2011

    Abreviews
    Application Western blot
    Sample Human Cell lysate - other (MCF7 breast cancer cells)
    Loading amount 20 µg
    Specification MCF7 breast cancer cells
    Treatment cells were transfected with siRNA against USP38
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"