Anti-USP38 antibody (ab72244)
Key features and details
- Rabbit polyclonal to USP38
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP38 antibody -
Description
Rabbit polyclonal to USP38 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Cow, Pig, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between residues 700 and 750 of human USP38 (NP_115946.2)
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Positive control
- Whole cell lysate from Hela and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) | |
IP |
Notes |
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WB: 1/2,000 - 1/10,000. Detects bands of approximately 31, 68, and 117 kDa (predicted molecular weight: 117 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Tissue specificity
Highly expressed in skeletal muscle. Expressed in adrenal gland. -
Sequence similarities
Belongs to the peptidase C19 family. - Information by UniProt
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Database links
- Entrez Gene: 84640 Human
- SwissProt: Q8NB14 Human
- Unigene: 480848 Human
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Alternative names
- KIAA1891 antibody
- Deubiquitinating enzyme 38 antibody
- HP43.8KD antibody
see all
Images
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All lanes : Anti-USP38 antibody (ab72244) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 117 kDa
Observed band size: 117 kDa
Additional bands at: 31 kDa, 68 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation/ Western Blot of USP38.
Lane 1: ab72244 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72244 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab72244 has been referenced in 2 publications.
- Wang Y et al. USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination. Viruses 13:N/A (2021). PubMed: 34696459
- Liu Q et al. Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses. Sci Adv 4:eaar2824 (2018). PubMed: 29732405