Overview

  • Product name
  • Description
    Rabbit polyclonal to USP4
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to C terminal of USP4 and derived from within residues 914-963 (Human)

  • Positive control
    • USP4-transfected HeLa cell lysate, Human hepatocarcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab38510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 108 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.
  • Tissue specificity
    Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.
  • Sequence similarities
    Belongs to the peptidase C19 family. USP4 subfamily.
    Contains 1 DUSP domain.
  • Post-translational
    modifications
    Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 4 antibody
    • MGC149848 antibody
    • MGC149849 antibody
    • Proto oncogene antibody
    • Protooncogene antibody
    • Ubiquitin carboxyl terminal hydrolase 4 antibody
    • Ubiquitin carboxyl-terminal hydrolase 4 antibody
    • Ubiquitin specific peptidase 4 (proto oncogene) antibody
    • Ubiquitin specific peptidase 4 (protooncogene) antibody
    • Ubiquitin specific peptidase 4 antibody
    • Ubiquitin specific processing protease 4 antibody
    • Ubiquitin specific protease 4 (proto oncogene) antibody
    • Ubiquitin specific protease 4 (protooncogene) antibody
    • Ubiquitin specific protease 4 antibody
    • Ubiquitin specific protease proto oncogene antibody
    • Ubiquitin specific protease protooncogene antibody
    • Ubiquitin thioesterase 4 antibody
    • Ubiquitin thiolesterase 4 antibody
    • Ubiquitin-specific-processing protease 4 antibody
    • Ubiquitous nuclear protein homolog antibody
    • UBP4_HUMAN antibody
    • UNP antibody
    • UNPH antibody
    • USP 4 antibody
    • USP4 antibody
    see all

Images

  • Anti-USP4 antibody (ab38510) + USP4-transfected HeLa cell lysate

    Predicted band size : 108 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Ab38510 staining Human hepatocarcinoma; formalin fixed, paraffin embedded. Ab38510 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

This product has been referenced in:
  • Li Y  et al. Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4. Int J Mol Med 38:803-11 (2016). Human . Read more (PubMed: 27430936) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab38510.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up