The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.
Tissue specificityOverexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.
Sequence similaritiesBelongs to the peptidase C19 family. USP4 subfamily. Contains 1 DUSP domain.
Post-translational modificationsMonoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
Ubiquitin specific peptidase 4 (proto oncogene) antibody
Ubiquitin specific peptidase 4 (protooncogene) antibody
Ubiquitin specific peptidase 4 antibody
Ubiquitin specific processing protease 4 antibody
Ubiquitin specific protease 4 (proto oncogene) antibody
Ubiquitin specific protease 4 (protooncogene) antibody
Ubiquitin specific protease 4 antibody
Ubiquitin specific protease proto oncogene antibody
Ubiquitin specific protease protooncogene antibody
Ubiquitin thioesterase 4 antibody
Ubiquitin thiolesterase 4 antibody
Ubiquitin-specific-processing protease 4 antibody
Ubiquitous nuclear protein homolog antibody
USP 4 antibody
Anti-USP4 antibody images
Immunoprecipitation - USP4 antibody (ab70617)
Detection of Human USP4 by Western Blot of Immunprecipitate.
ab70617 at 1µg/ml staining USP4 (approximately 120kDa) in HeLa whole cell lysates immunoprecipitated using ab70617 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-USP4 antibody (ab70617)
has not yet been referenced specifically in any publications.
Publishing research using ab70617? Please let us know so that we can cite the reference in this datasheet.
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