Overview

  • Product name
  • Description
    Rabbit polyclonal to USP4
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 225 and 275 of human USP4

  • Positive control
    • HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.

    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.
    • Tissue specificity
      Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.
    • Sequence similarities
      Belongs to the peptidase C19 family. USP4 subfamily.
      Contains 1 DUSP domain.
    • Post-translational
      modifications
      Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.
    • Cellular localization
      Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 4 antibody
      • MGC149848 antibody
      • MGC149849 antibody
      • Proto oncogene antibody
      • Protooncogene antibody
      • Ubiquitin carboxyl terminal hydrolase 4 antibody
      • Ubiquitin carboxyl-terminal hydrolase 4 antibody
      • Ubiquitin specific peptidase 4 (proto oncogene) antibody
      • Ubiquitin specific peptidase 4 (protooncogene) antibody
      • Ubiquitin specific peptidase 4 antibody
      • Ubiquitin specific processing protease 4 antibody
      • Ubiquitin specific protease 4 (proto oncogene) antibody
      • Ubiquitin specific protease 4 (protooncogene) antibody
      • Ubiquitin specific protease 4 antibody
      • Ubiquitin specific protease proto oncogene antibody
      • Ubiquitin specific protease protooncogene antibody
      • Ubiquitin thioesterase 4 antibody
      • Ubiquitin thiolesterase 4 antibody
      • Ubiquitin-specific-processing protease 4 antibody
      • Ubiquitous nuclear protein homolog antibody
      • UBP4_HUMAN antibody
      • UNP antibody
      • UNPH antibody
      • USP 4 antibody
      • USP4 antibody
      see all

    Images

    • Detection of Human USP4 by Western Blot of Immunprecipitate. ab70617 at 1µg/ml staining USP4 (approximately 120kDa) in HeLa whole cell lysates immunoprecipitated using ab70617 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab70617 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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