Recombinant Anti-USP4 antibody [EPR13846] (ab181105)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13846] to USP4
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-USP4 antibody [EPR13846]
See all USP4 primary antibodies -
Description
Rabbit monoclonal [EPR13846] to USP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HuT-78 and THP1 cell lysates,
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13846 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 109 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000. Predicted molecular weight: 109 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. -
Tissue specificity
Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons. -
Sequence similarities
Belongs to the peptidase C19 family. USP4 subfamily.
Contains 1 DUSP domain. -
Post-translational
modificationsMonoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type. - Information by UniProt
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Database links
- Entrez Gene: 7375 Human
- Omim: 603486 Human
- SwissProt: Q08AK7 Human
- SwissProt: Q08AK8 Human
- SwissProt: Q13107 Human
- Unigene: 403828 Human
- Unigene: 77500 Human
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Alternative names
- Deubiquitinating enzyme 4 antibody
- MGC149848 antibody
- MGC149849 antibody
see all
Images
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Immunofluorescent staining of -20℃ Acetone-fixed THP1 cells labeling USP4 using ab181105 at a 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/200 dilution and counter stained with DAPI.
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All lanes : Anti-USP4 antibody [EPR13846] (ab181105) at 1/1000 dilution
Lane 1 : HuT-78 cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181105 has been referenced in 2 publications.
- Wang Y et al. Ubiquitin-specific protease 4 predicts an unfavorable prognosis and promotes malignant behaviors in vitro in pancreatic cancer. Exp Cell Res 396:112317 (2020). PubMed: 33038351
- Guo W et al. Up-regulated deubiquitinase USP4 plays an oncogenic role in melanoma. J Cell Mol Med 22:2944-2954 (2018). PubMed: 29542252