Overview

  • Product nameAnti-USP40 antibody
  • Description
    Rabbit polyclonal to USP40
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 755-900 of Human USP40.

  • Positive control
    • Human tonsil tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab121234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use Buffer at pH 6 for antigen retrieval.

Target

  • FunctionMay be catalytically inactive.
  • Tissue specificityBroadly expressed.
  • Sequence similaritiesBelongs to the peptidase C19 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 40 antibody
    • Ubiquitin carboxyl-terminal hydrolase 40 antibody
    • Ubiquitin thiolesterase 40 antibody
    • Ubiquitin-specific-processing protease 40 antibody
    • UBP40_HUMAN antibody
    • Usp40 antibody
    see all

Anti-USP40 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles and focal adhesions. Recommended concentration of ab121234 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121234, at 1/75 dilution, staining USP40 in Human tonsil tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Note: shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.

References for Anti-USP40 antibody (ab121234)

ab121234 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - other (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jan 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"