Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab121254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Function
    Deubiquitinating enzyme which may play an important role during spermatogenesis.
  • Tissue specificity
    Broadly expressed.
  • Sequence similarities
    Belongs to the peptidase C19 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 42 antibody
    • FLJ12697 antibody
    • Ubiquitin carboxyl-terminal hydrolase 42 antibody
    • ubiquitin specific peptidase 42 antibody
    • Ubiquitin thiolesterase 42 antibody
    • Ubiquitin-specific-processing protease 42 antibody
    • UBP42 antibody
    • UBP42_HUMAN antibody
    • USP42 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121254 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical staining of parffin embedded Human kidney, using 1/60 ab121254, shows strong nuclear cytoplasmic positivity in cells in tubules and cells in glomeruli.

References

ab121254 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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