Overview

  • Product nameAnti-USP47 antibody
  • Description
    Rabbit polyclonal to USP47
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human USP47 (NP_060414.3)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceUSP47 belongs to the peptidase C19 family and may be catalytically inactive although it seems to have kept all necessary active site residues. It is expressed in skeletal muscle, heart and testis and there are 3 named isoforms produced by alternative splicing.
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 47 antibody
      • TRF (TATA bidning protein-related factor) proximal homolog antibody
      • TRFP antibody
      • Ubiquitin carboxyl terminal hydrolase 47 antibody
      • ubiquitin specific peptidase 47 antibody
      • Ubiquitin specific processing protease 47 antibody
      • Ubiquitin thioesterase 47 antibody
      • USP 47 antibody
      see all

    Anti-USP47 antibody images

    • All lanes : Anti-USP47 antibody (ab72143) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg


      Predicted band size : 158 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds
    • Detection of USP47 by Western Blot of Immunprecipitate.
      ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References for Anti-USP47 antibody (ab72143)

    This product has been referenced in:
    • Sako-Kubota K  et al. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell 25:3851-60 (2014). Read more (PubMed: 25253721) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"