Overview

  • Product nameAnti-USP5 antibody
    See all USP5 primary antibodies
  • Description
    Mouse monoclonal to USP5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 71-181 of Human USP5

Properties

Applications

Our Abpromise guarantee covers the use of ab56372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
    • Sequence similaritiesBelongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • DomainThe UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 5 antibody
      • Isopeptidase T antibody
      • ISOT 1 antibody
      • ISOT antibody
      • Ubiquitin carboxyl-terminal hydrolase 5 antibody
      • Ubiquitin isopeptidase T antibody
      • Ubiquitin specific peptidase 5 (isopeptidase T) antibody
      • Ubiquitin specific peptidase 5 antibody
      • Ubiquitin specific processing protease 5 antibody
      • Ubiquitin specific protease 5 (isopeptidase T) antibody
      • Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody
      • Ubiquitin thioesterase 5 antibody
      • Ubiquitin thiolesterase 5 antibody
      • Ubiquitin-specific-processing protease 5 antibody
      • UBP5_HUMAN antibody
      • Usp5 antibody
      see all

    Anti-USP5 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56372 USP5 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

    References for Anti-USP5 antibody (ab56372)

    ab56372 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"