Overview

  • Product name
    Anti-USP5 antibody [EPR10454]
    See all USP5 primary antibodies
  • Description
    Rabbit monoclonal [EPR10454] to USP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human USP5 (UniProt: P45974).

  • Positive control
    • MCF7, A549, Mouse brain, Rat brain and Rat heart lysates; MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
    • Sequence similarities
      Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • Domain
      The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 5 antibody
      • Isopeptidase T antibody
      • ISOT 1 antibody
      • ISOT antibody
      • Ubiquitin carboxyl-terminal hydrolase 5 antibody
      • Ubiquitin isopeptidase T antibody
      • Ubiquitin specific peptidase 5 (isopeptidase T) antibody
      • Ubiquitin specific peptidase 5 antibody
      • Ubiquitin specific processing protease 5 antibody
      • Ubiquitin specific protease 5 (isopeptidase T) antibody
      • Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody
      • Ubiquitin thioesterase 5 antibody
      • Ubiquitin thiolesterase 5 antibody
      • Ubiquitin-specific-processing protease 5 antibody
      • UBP5_HUMAN antibody
      • Usp5 antibody
      see all

    Images

    • All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 96 kDa

    • All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Rat brain lysates
      Lane 3 : Rat heart lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 96 kDa

    • Immunofluorescent analysis of MCF7 cells labeling USP5 with ab154170 at 1/50 dilution.

    References

    This product has been referenced in:
    • Esquerdo M  et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . Read more (PubMed: 26934049) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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