Overview

  • Product name
  • Description
    Rabbit polyclonal to USP50
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide within Human USP50 aa 148-178 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q70EL3

  • Positive control
    • IHC-P: Human skeletal muscle tissue. WB: Y79 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.

Target

  • Function
    Has no peptidase activity.
  • Tissue specificity
    Weakly expressed in a few tissues.
  • Sequence similarities
    Belongs to the peptidase C19 family.
    Contains 1 USP domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Inactive ubiquitin carboxyl-terminal hydrolase 50 antibody
    • Inactive ubiquitin specific peptidase 50 antibody
    • Inactive ubiquitin-specific peptidase 50 antibody
    • Ubiquitin specific peptidase 50 antibody
    • Ubiquitin specific protease 50 antibody
    • UBP50_HUMAN antibody
    • Usp50 antibody
    see all

Images

  • Anti-USP50 antibody (ab170342) at 1/100 dilution + Y79 cell line lysate at 35 µg

    Predicted band size: 39 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle labeling USP50 with ab170342 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

References

ab170342 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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