Overview

  • Product nameAnti-USP51 antibody
    See all USP51 primary antibodies
  • Description
    Rabbit polyclonal to USP51
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Pig, Gorilla
  • Immunogen

    Recombinant fragment: LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEP, corresponding to internal sequence amino acids 211-343 of Human USP51 (UniProt ID: Q70EK9).

  • Positive control
    • IHC-P: Human pancreas tissue WB: Human RT-4, U-251 MG, A-431, liver, and tonsil lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab121147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificityExpressed in prostate, brain, lung, aorta and kidney.
  • Sequence similaritiesBelongs to the peptidase C19 family.
    Contains 1 UBP-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 51 antibody
    • Ubiquitin carboxyl-terminal hydrolase 51 antibody
    • Ubiquitin specific peptidase 51 antibody
    • Ubiquitin thioesterase 51 antibody
    • Ubiquitin thiolesterase 51 antibody
    • Ubiquitin-specific-processing protease 51 antibody
    • UBP51_HUMAN antibody
    • USP51 antibody
    see all

Anti-USP51 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-USP51 antibody (ab121147) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : A-431 cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab121147 at a 1/100 dilution. The image shows strong cytoplasmic positivity in exocrine glandular cells.

References for Anti-USP51 antibody (ab121147)

ab121147 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"