Overview

  • Product nameAnti-USP6 antibody
    See all USP6 primary antibodies
  • Description
    Mouse monoclonal to USP6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: DMVENADSLQ AQERKDILMK YDKGHRAGLP EDKGPEPVGI NSSIDRFGIL HETELPPVTA REAKKIRREM TRTSKWMEML GEWETYKH, corresponding to amino acids 2-90 of Human USP6

Properties

Applications

Our Abpromise guarantee covers the use of ab56431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDeubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
    • Tissue specificityTestis specific. Expressed in various cancer cell lines.
    • Involvement in diseaseNote=A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation.
    • Sequence similaritiesBelongs to the peptidase C19 family.
      Contains 1 Rab-GAP TBC domain.
    • DomainThe Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6.
    • Post-translational
      modifications
      Monubiquitinated; ubiquitination is calmodulin and calcium dependent.
    • Cellular localizationCell membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 6 antibody
      • HRP1 antibody
      • Proto-oncogene TRE-2 antibody
      • TRE17 antibody
      • TRE2 antibody
      • Ubiquitin carboxyl-terminal hydrolase 6 antibody
      • Ubiquitin specific protease 6 antibody
      • Ubiquitin thiolesterase 6 antibody
      • Ubiquitin-specific-processing protease 6 antibody
      • UBP6_HUMAN antibody
      • USP6 antibody
      see all

    Anti-USP6 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56431 USP6 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

    References for Anti-USP6 antibody (ab56431)

    ab56431 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Tissue lysate - other (Brain TBS, SDS & Urea fractions)
    Loading amount 20 µg
    Specification Brain TBS, SDS & Urea fractions
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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    Submitted Nov 07 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"