The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionDeubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.
Tissue specificityWidely expressed in embryonic and adult tissues.
Sequence similaritiesBelongs to the peptidase C19 family.
ab111046, at 3.75 ug/ml, staining USP9x in formalin-fixed, paraffin-embedded Human brain cortex tissue. Stained using a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen
References for Anti-USP9x antibody (ab111046)
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