FunctionDeubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.
Tissue specificityWidely expressed in embryonic and adult tissues.
Sequence similaritiesBelongs to the peptidase C19 family.
Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila) antibody
X chromosome antibody
Anti-USP9x antibody images
Western blot - USP9x antibody (ab99343)
All lanes : Anti-USP9x antibody (ab99343) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 289 kDa
Exposure time : 30 seconds
Immunoprecipitation - USP9x antibody (ab99343)
Detection of USP9x in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99343 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-USP9x antibody (ab99343)
This product has been referenced in:
Paemka L et al. Seizures are regulated by ubiquitin-specific peptidase 9 X-linked (USP9X), a de-ubiquitinase. PLoS Genet11:e1005022 (2015).
Read more (PubMed: 25763846) »
Oosterkamp HM et al. USP9X downregulation renders breast cancer cells resistant to tamoxifen. Cancer Res74:3810-20 (2014).
Read more (PubMed: 25028367) »