Overview

  • Product nameAnti-USP9x antibody
    See all USP9x primary antibodies
  • Description
    Rabbit polyclonal to USP9x
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 625-675 of Human USP9x (CAD18900.2).

  • Positive control
    • HeLa, 293T and NIH 3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 289 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionDeubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.
  • Tissue specificityWidely expressed in embryonic and adult tissues.
  • Sequence similaritiesBelongs to the peptidase C19 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme FAF X antibody
    • Deubiquitinating enzyme FAF-X antibody
    • DFFRX antibody
    • Drosophila fat facets related X linked antibody
    • FAF antibody
    • Fafl antibody
    • Fam antibody
    • Fat facets homolog antibody
    • Fat facets in mammals antibody
    • Fat facets protein related X linked antibody
    • Fat facets protein related, X-linked antibody
    • Fat facets protein-related antibody
    • hFAM antibody
    • MRX99 antibody
    • Probable ubiquitin carboxyl terminal hydrolase FAF X antibody
    • Probable ubiquitin carboxyl-terminal hydrolase FAF-X antibody
    • Ubiquitin carboxyl-terminal hydrolase FAM antibody
    • Ubiquitin specific peptidase 9 X linked antibody
    • Ubiquitin specific peptidase 9, X-linked antibody
    • Ubiquitin specific processing protease FAF X antibody
    • Ubiquitin specific protease 9 X chromosome antibody
    • Ubiquitin thioesterase FAF X antibody
    • Ubiquitin thiolesterase FAF X antibody
    • Ubiquitin thiolesterase FAF-X antibody
    • Ubiquitin-specific protease 9 antibody
    • Ubiquitin-specific-processing protease FAF-X antibody
    • USP9 (gene name) antibody
    • Usp9x antibody
    • USP9X_HUMAN antibody
    • Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila) antibody
    • X chromosome antibody
    • X-linked antibody
    see all

Anti-USP9x antibody images

  • All lanes : Anti-USP9x antibody (ab99343) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 289 kDa


    Exposure time : 30 seconds
  • Detection of USP9x in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99343 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-USP9x antibody (ab99343)

This product has been referenced in:
  • Paemka L  et al. Seizures are regulated by ubiquitin-specific peptidase 9 X-linked (USP9X), a de-ubiquitinase. PLoS Genet 11:e1005022 (2015). Read more (PubMed: 25763846) »
  • Oosterkamp HM  et al. USP9X downregulation renders breast cancer cells resistant to tamoxifen. Cancer Res 74:3810-20 (2014). WB ; Human . Read more (PubMed: 25028367) »

See all 2 Publications for this product

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