Overview

  • Product name
  • Description
    Rabbit polyclonal to Uteroglobin
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC/IF, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant rat uteroglobin His fusion protein.

  • General notes


    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Primary antibody notes
    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1600.
ELISA Use at an assay dependent concentration. PubMed: 21787397
ICC/IF Use at an assay dependent concentration. PubMed: 24367567
WB 1/5000. Predicted molecular weight: 10 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • Tissue specificity
    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • Sequence similarities
    Belongs to the secretoglobin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blastokinin antibody
    • CC10 antibody
    • CC16 antibody
    • CCPBP antibody
    • CCSP antibody
    • Clara cell phospholipid binding protein antibody
    • Clara cell phospholipid-binding protein antibody
    • Clara cell specific 10 kD protein antibody
    • Clara cells 10 kDa secretory protein antibody
    • OTTHUMP00000236107 antibody
    • SCGB1A1 antibody
    • Secretoglobin family 1A member 1 antibody
    • Secretoglobin, family 1A, member 1 (uteroglobin) antibody
    • UG antibody
    • UGB antibody
    • UP-1 antibody
    • UP1 antibody
    • Urinary protein 1 antibody
    • Urine protein 1 antibody
    • UTER_HUMAN antibody
    • Uteroglobin antibody
    see all

Anti-Uteroglobin antibody images

  • Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).

  • ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image. 

  • All lanes : Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution

    Lane 1 : Mouse lung whole tissue lysate with Milk
    Lane 2 : Mouse kidney whole tissue lysate with Milk

    Lysates/proteins at 20 µg per lane.
    Blocking peptides at 5 % per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

References for Anti-Uteroglobin antibody (ab40873)

This product has been referenced in:
  • Strupp C  et al. Relationship of Metabolism and Cell Proliferation to the Mode of Action of Fluensulfone-Induced Mouse Lung Tumors. II: Additional Mechanistic Studies. Toxicol Sci 154:296-308 (2016). Read more (PubMed: 27605420) »
  • Crabbé A  et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One 10:e0126846 (2015). IHC-P ; Mouse . Read more (PubMed: 25962111) »

See all 14 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Feb 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung, bronchial epithelium)
Permeabilization
No
Specification
lung, bronchial epithelium
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Dec 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (urine)
Loading amount
5 µg
Specification
urine
Treatment
CLB (NP-40 based lysis buffer with protease inhibitors) , 10 min on ice
Gel Running Conditions
Reduced Denaturing (18)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Lung Parenchyma)
Specification
Human Lung Parenchyma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
1% BSA with 0.1% Triton-X in PBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 18°C
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Verified customer

Submitted Mar 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin (Ab970) 0.37%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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