Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).
Immunohistochemistry (Frozen sections) - Uteroglobin antibody (ab40873)Image from Nyeng P et. al., BMC Dev Biol. 2008; 8: 2 (Fig. 6D)
ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image.
Western blot - Uteroglobin antibody (ab40873)
Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution + Wild-type adult mouse lung homogenate was used.
Secondary 5% Dry milk, 1 hour at room temperature at 1/5000 dilution
Predicted band size : 10 kDa Observed band size : 10 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Uteroglobin antibody (ab40873)Image courtesy of an anonymous Abreview.
ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.