Anti-UTF1 antibody (ab105090)

Overview

  • Product name
  • Description
    Rabbit polyclonal to UTF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human UTF1.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: E14Tg2a; F9; IOUD2.

Properties

Applications

Our Abpromise guarantee covers the use of ab105090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • Function
    Acts as a transcriptional coactivator of ATF2.
  • Domain
    The leucine-zipper domain is required for coactivation activity. When this domain is deleted, the protein is able to stimulate transcription from a number of gene promoters.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.
  • Alternative names
    • hUTF 1 antibody
    • hUTF1 antibody
    • Undifferentiated embryonic cell transcription factor 1 antibody
    • UTF 1 antibody
    • UTF1 antibody
    • UTF1_HUMAN antibody
    see all

Images

  • All lanes : Anti-UTF1 antibody (ab105090) at 1 µg/ml

    Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 36 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    The expression profile observed is consistent with what has been described in the literature (PMID:17785516).
  • ICC/IF image of ab105090 stained mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) mouse ES cells at 1µg/ml.

References

ab105090 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Expiration date: January 11th. 2013

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