FormUTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.
ICC/IF image of ab24273 stained mouse embryonic stem cells. The cells were PFA fixed (4% PFA, 20 min) and incubated with the antibody (ab24273, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
The large nuclei of the feeder cells can be seen in the image; ab24273 does not localise to these nuclei. ab24273 can be seen localising to the much smaller nuclei of the mouse embryonic stem cells.
Western blot - UTF1 antibody (ab24273)
All lanes : Anti-UTF1 antibody (ab24273) at 1 µg/ml
Lane 1 : Mouse Embryonic Stem Cell Lysate Lane 2 : Mouse Embryonic Stem Cell Lysate with Human UTF1 peptide (ab30369) at 1 µg/ml