Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab36655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Can be blocked with Human UTY peptide (ab36963).

Target

  • FunctionHistone demethylase.
  • Sequence similaritiesBelongs to the UTX family.
    Contains 1 JmjC domain.
    Contains 8 TPR repeats.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession O14607 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Histone demethylase UTY antibody
    • Ubiquitously transcribed TPR protein on the Y chromosome antibody
    • Ubiquitously transcribed TPR protein on the Y chromosome antibody
    • Ubiquitously transcribed Y chromosome tetratricopeptide repeat protein antibody
    • Ubiquitously-transcribed TPR protein on the Y chromosome antibody
    • Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein antibody
    • UTY antibody
    • UTY_HUMAN antibody
    see all

Anti-UTY antibody images

  • IHC image of UTY staining in human lymphoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36655, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-UTY antibody (ab36655)

ab36655 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Human and mouse cell lysate (see figure))
Loading amount 20 µg
Specification Human and mouse cell lysate (see figure)
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step 5% milk in PBS + 0.1% Tween as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"