Overview

  • Product name
  • Description
    Rabbit polyclonal to UTY
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 775 and 825 of human UTY (NP_009056.3)

  • Positive control
    • Jurkat whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab85969 was affinity purified using an epitope specific to UTY immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Histone demethylase.
    • Sequence similarities
      Belongs to the UTX family.
      Contains 1 JmjC domain.
      Contains 8 TPR repeats.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Histone demethylase UTY antibody
      • Ubiquitously transcribed TPR protein on the Y chromosome antibody
      • Ubiquitously transcribed Y chromosome tetratricopeptide repeat protein antibody
      • Ubiquitously-transcribed TPR protein on the Y chromosome antibody
      • Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein antibody
      • UTY antibody
      • UTY_HUMAN antibody
      see all

    Images

    • All lanes : Anti-UTY antibody (ab85969) at 0.04 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : Jurkat whole cell lysate at 5 µg


      Predicted band size : 150 kDa
      Observed band size : 165 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of UTY by Western Blot of Immunprecipitate.
      ab85969 at 1µg/ml staining UTY in Jurkat whole cell lysate immunoprecipitated using ab85969 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab85969 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you very much for your interest in ab85969.

    To our knowledge, this product has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab85969 now, test it in IHC and submit feedback to us in the fo...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up