Overview

  • Product nameAnti-UTY antibody
    See all UTY primary antibodies
  • Description
    Rabbit polyclonal to UTY
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 775 and 825 of human UTY (NP_009056.3)

  • Positive control
    • Jurkat whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab85969 was affinity purified using an epitope specific to UTY immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab85969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHistone demethylase.
    • Sequence similaritiesBelongs to the UTX family.
      Contains 1 JmjC domain.
      Contains 8 TPR repeats.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Histone demethylase UTY antibody
      • Ubiquitously transcribed TPR protein on the Y chromosome antibody
      • Ubiquitously transcribed Y chromosome tetratricopeptide repeat protein antibody
      • Ubiquitously-transcribed TPR protein on the Y chromosome antibody
      • Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein antibody
      • UTY antibody
      • UTY_HUMAN antibody
      see all

    Anti-UTY antibody images

    • All lanes : Anti-UTY antibody (ab85969) at 0.04 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : Jurkat whole cell lysate at 5 µg


      Predicted band size : 150 kDa
      Observed band size : 165 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of UTY by Western Blot of Immunprecipitate.
      ab85969 at 1µg/ml staining UTY in Jurkat whole cell lysate immunoprecipitated using ab85969 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    References for Anti-UTY antibody (ab85969)

    ab85969 has not yet been referenced specifically in any publications.

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