Overview

  • Product nameAnti-UVRAG antibody
    See all UVRAG primary antibodies
  • Description
    Rabbit polyclonal to UVRAG
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 649-699 of Human UVRAG (NP_003360.2; GeneID 7405).

  • Positive control
    • Human Controls: Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma Mouse Controls: Renal Cell Carcinoma, Teratoma

Properties

Applications

Our Abpromise guarantee covers the use of ab105114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.

Target

  • FunctionVersatile protein that is involved in regulation of differenent cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:24056303). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300).
    Involved in maintaining chromosomal stability. Promotes DNA double-strand break (DSB) repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining (NHEJ) (PubMed:22542840). Required for centrosome stability and proper chromosome segregation (PubMed:22542840).
  • Tissue specificityHighly expressed in brain, lung, kidney and liver.
  • Involvement in diseaseA chromosomal aberration involving UVRAG has been observed in a patient with heterotaxy (left-right axis malformation). Inversion Inv(11)(q13.5;q25).
  • Sequence similaritiesContains 1 C2 domain.
  • Post-translational
    modifications
    Phosphorylated at Ser-498 by MTOR under basal conditions; increases the interaction with RUBCN implicated in inhibitory effect of RUBCN on PI3KC3 and decreases interaction with RAB7,A and VPS16 and VPS39 (indicative for a class C Vps complex, possibly the HOPS complex) (PubMed:25533187).
  • Cellular localizationLate endosome. Lysosome. Early endosome. Endoplasmic reticulum. Midbody. Chromosome, centromere. Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network. Colocalization with early endosomes is only partial.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHTX antibody
    • Disrupted in heterotaxy antibody
    • p63 antibody
    • UV radiation resistance associated antibody
    • UV radiation resistance associated gene antibody
    • UV radiation resistance associated gene protein antibody
    • UV radiation resistance-associated gene protein antibody
    • UVRAG antibody
    • UVRAG_HUMAN antibody
    see all

Anti-UVRAG antibody images

  • ab105114, at a 1/250 dilution, staining UVRAG in formalin fixed, paraffin embedded Human Colon Carcinoma tissue by Immunohistochemistry. Detection: DAB staining
  • ab105114, at a 1/250 dilution, staining UVRAG in formalin fixed, paraffin embedded Mouse Renal Cell Carcinoma tissue by Immunohistochemistry. Detection: DAB staining

References for Anti-UVRAG antibody (ab105114)

ab105114 has not yet been referenced specifically in any publications.

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