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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Anti-v-Myb + c-Myb (phospho S12) antibody
See all v-Myb + c-Myb products (6) ...
Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.
WB, ELISA, IHC-Pmore details
Reacts with
Human
Predicted to work with
Mouse
Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).
Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Polyclonal
IgG
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Our Abpromise guarantee covers the use of ab78892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 72 kDa.
ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
Nuclear
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892)

Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892)

All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with Hu (2nM, 24hours)
Lane 2 : Extracts from HeLa cells treated
with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 72 kDa
Observed band size : 72 kDa
ab78892 has not yet been referenced specifically in any publications.
Publishing research using ab78892? Please let us know so that we can cite the reference in this datasheet
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.

All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with Hu (2nM, 24hours)
Lane 2 : Extracts from HeLa cells treated
with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 72 kDa
Observed band size : 72 kDa
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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