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Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

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Overview

Product name

Anti-v-Myb + c-Myb (phospho S12) antibody
See all v-Myb + c-Myb products (6) ...

Description

Rabbit polyclonal to v-Myb + c-Myb (phospho S12)

Specificity

ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).

Positive control

Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892) image (enlarge)

  • Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892)Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab78892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 72 kDa.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Relevance

Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.

Cellular localization

Nuclear

Alternative names

  • C myb antibody
  • C myb protein antibody
  • Cmyb antibody
  • MYB antibody
  • Myb proto oncogene antibody
  • V myb myeloblastosis viral oncogene homolog antibody
see all

Database links

Anti-v-Myb + c-Myb (phospho S12) antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892)

Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.

  Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892)

Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892)

All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution

Lane 1 : Extracts from HeLa cells treated with Hu (2nM, 24hours)
Lane 2 : Extracts from HeLa cells treated with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 72 kDa
Observed band size : 72 kDa

References for Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

ab78892 has not yet been referenced specifically in any publications.

Publishing research using ab78892? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"