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Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

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Overview

  • Product nameAnti-v-Myb + c-Myb (phospho S12) antibodySee all v-Myb + c-Myb primary antibodies ...
  • Description
    Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
  • Specificityab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).

  • Positive controlHuman heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab78892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 72 kDa.
ELISA ELISA: 1/5000.
IHC-P IHC-P: 1/50 - 1/100.

Target

  • RelevanceTranscriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
        C myb antibodyC myb protein antibodyCmyb antibody
        MYB antibodyMyb proto oncogene antibodyV myb myeloblastosis viral oncogene homolog antibody
      see all

    Anti-v-Myb + c-Myb (phospho S12) antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
    • Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892)
      All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution

      Lane 1 : Extracts from HeLa cells treated with Hu (2nM, 24hours)
      Lane 2 : Extracts from HeLa cells treated with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 72 kDa
      Observed band size : 72 kDa

    References for Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

    ab78892 has not yet been referenced specifically in any publications.

    Publishing research using ab78892 ? Please let us know so that we can cite the reference in this datasheet

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