Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
Key features and details
- Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-v-Myb + c-Myb (phospho S12) antibody -
Description
Rabbit polyclonal to v-Myb + c-Myb (phospho S12) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human v-Myb + c-Myb (phospho S12).
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Positive control
- Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Relevance
Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Omim: 189990 Human
- SwissProt: P10242 Human
- SwissProt: P06876 Mouse
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Alternative names
- C myb antibody
- C myb protein antibody
- Cmyb antibody
see all
Images
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
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All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with Hu (2nM, 24hours)
Lane 2 : Extracts from HeLa cells treated
with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab78892 has not yet been referenced specifically in any publications.