Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150)

Overview

  • Product nameAnti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade
    See all v-Myb + c-Myb primary antibodies
  • Description
    Rabbit monoclonal [EP769Y] to v-Myb + c-Myb (phospho S11) - ChIP Grade
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, ChIPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human c-Myb.

  • Positive control
    • IHC-P: human cervical carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab45150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
IP 1/50.
ChIP Use at an assay dependent concentration. PubMed: 21296997
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • RelevanceTranscriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • C myb antibody
      • C myb protein antibody
      • Cmyb antibody
      • MYB antibody
      • Myb proto oncogene antibody
      • V myb myeloblastosis viral oncogene homolog antibody
      see all

    Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade images

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/2000 dilution (purified)

      Lane 1 : Molt-4 cell lysate with v-Myb + c-Myb (phospho S11) peptide
      Lane 2 : Molt-4 cell lysate with v-Myb + c-Myb (phospho S12) peptide
      Lane 3 : Molt-4 cell lysate with v-Myb + c-Myb (phospho S11/12) peptide
      Lane 4 : Molt-4 cell lysate with v-Myb + c-Myb unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/2000 dilution (purified)

      Lane 1 : HeLa cell lysate with v-Myb + c-Myc (phospho S11) peptide
      Lane 2 : HeLa cell lysate with v-Myb + c-Myc (phospho S12) peptide
      Lane 3 : HeLa cell lysate with v-Myb + c-Myc (phospho S11/12) peptide
      Lane 4 : HeLa cell lysate with v-Myb + c-Myc unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size : 72 kDa
      Observed band size : 75,77 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/5000 dilution (purified)

      Lane 1 : Untreated Ramos cell lysate
      Lane 2 : Ramos cell lysate, membrane treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 75,77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Dot blot analysis of v-Myb + c-Myb (phospho S11/12) peptide (Lane 1) and v-Myb + c-Myb unmodified peptide (Lane 2) labelling v-Myb + c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/5000 dilution (purified)

      Lane 1 : Untreated HL-60 cell lysate
      Lane 2 : HL-60 cell lysate, membrane treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/2000 dilution (purified)

      Lane 1 : untreated HeLa cell lysate
      Lane 2 : HeLa cell lysate, the membrane treated with lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size : 72 kDa
      Observed band size : 75,77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/10000 dilution (unpurified) + Ramos membrane

      Predicted band size : 72 kDa
      Observed band size : 72,75 kDa (why is the actual band size different from the predicted?)
    • Unpurified ab45150 staining human v-Myb / c-Myb in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.

    References for Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150)

    This product has been referenced in:
    • Aisagbonhi OA  et al. Fine-Needle Aspiration of Epithelial-Myoepithelial Carcinoma of the Parotid Gland With Prominent Adenoid Cystic Carcinoma-Like Cribriform Features: Avoiding a Diagnostic Pitfall. Am J Clin Pathol N/A:N/A (2016). Read more (PubMed: 27614667) »
    • Costa AF  et al. Analysis of MYB oncogene in transformed adenoid cystic carcinomas reveals distinct pathways of tumor progression. Lab Invest 94:692-702 (2014). Human . Read more (PubMed: 24732452) »

    See all 12 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (chronic myelogenous leukemia & monkey kidney cells)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris (BioRad))
    Loading amount 50000 cells
    Specification chronic myelogenous leukemia & monkey kidney cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Ragnhild Eskeland

    Verified customer

    Submitted Apr 22 2015

    Thank you for contacting us.

    This product is raised against the N-terminal region. It should not be affected by a truncated C-terminus. We do guarantee this to recognizeAvian myeloblastosis viral (v Myb) in human in the following applicati...

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    Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

    Die Protokolldetails bestätigen meine Vermutung, dass der anti-Myb-Antikörper wohl leider fehlerhaft ist. Zwar ist die Lyse mit Ultraschall vielleicht etwa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"