Overview

  • Product name
  • Description
    Chicken polyclonal to V5 tag
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide:

    GKPIPNPLLGLDST(C)

    (V5 epitope) conjugated to KLH. (Antibody raised against peptide available as ab15829.)

  • Positive control
    • This antibody gave a positive signal in Drosophila S2 cells transfected with V5 epitope tagged protein. No band was observed in the negative control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 3% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab107244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Block using 3% milk.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • All lanes : Anti-V5 tag antibody (ab107244) at 1 µg/ml

    Lane 1 : Drosophila S2 cells transfected with V5 epitope tagged MSI-A
    Lane 2 : Drosophila S2 Negative Control

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

References

ab107244 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab107244.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up