• Product nameAnti-V5 tag antibody
    See all V5 tag primary antibodies
  • Description
    Rabbit polyclonal to V5 tag
  • Tested applicationsSuitable for: ChIP, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Parainfluenza virus 5 V5 tag aa 50-150 conjugated to keyhole limpet haemocyanin.
    Database link: P11207
    (Peptide available as ab15829)

  • Positive control
    • This antibody gave a positive signal in Western Blot when tested against a V5-tagged protein.



Our Abpromise guarantee covers the use of ab15828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
IP Use at an assay dependent dilution. PubMed: 20850014
WB Use a concentration of 0.5 µg/ml.


  • RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody images

  • A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
  • All lanes : Anti-V5 tag antibody (ab15828) at 0.5 µg/ml

    Lane 1 : Recombinant V5 tagged Groucho homolog
    Lane 2 : Recombinant V5 tagged Groucho with V5 tag peptide (ab15829) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Goat anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-V5 tag antibody (ab15828)

This product has been referenced in:
  • Iempridee T  et al. Epstein-Barr virus utilizes Ikaros in regulating its latent-lytic switch in B cells. J Virol 88:4811-27 (2014). ChIP . Read more (PubMed: 24522918) »
  • Wang Y  et al. ASPP2 controls epithelial plasticity and inhibits metastasis through ß-catenin-dependent regulation of ZEB1. Nat Cell Biol 16:1092-104 (2014). Read more (PubMed: 25344754) »

See all 11 Publications for this product

Product Wall

I am sorry that this product has been giving results which have made analysis difficult. I am happy to provide the blocking peptide for this product free of charge, so that you might run an immunogen blocking experiment to determine specificity. I hav...

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I'm sorry but there is no information available regarding how many V5 tags were incorporated into this GAL4 fusion protein.

Please contact us againif you have any further questions.

Thanks for your enquiry.

The scientist who may have further information regarding the number of V5 tags on the vector is out of town until next Monday. I will follow up with you early next week.

Thanks for your patience! Please feel...

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Thank you for your inquiry.
I would like to reassure you that both antibodies are tested and guaranteed for CHIP.
I can confirm that ab15828 was tested by our lab in house for CHIP and therefore I would recommend to use this antibody.

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab15828. To check the status of the order please contact our Customer...

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Thank you for letting me know the progress. It does look like the expression in the HeLa cell line is significantly better and the background staining does appear to have been reduced considerably. I can however understand your concern in continuing to...

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As far as I could tell, expression in HeLa cells should not be a problem with the CMV promoter so if you still get the same results after trying this get back to me and we'll discuss what the next course of action is (refund/alternative antibody). I...

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Thank you for taking time to complete our questionnaire. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some sugge...

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. As discussed, I a...

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Thank you for your enquiry. We currently have 6 antibodies against the major tags that have all been validated and shown to work by chromatin immunoprecipitation. Our HA antiserum was shown to work by ChIP using Xenopus oocytes transfected with an H...

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