Overview

  • Product name
  • Description
    Goat polyclonal to V5 tag
  • Specificity
    Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, ICC, IHC - Wholemountmore details
  • Immunogen

    Synthetic peptide:

    GKPIPNPLLGLDST

    (V5) conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/30000. Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent concentration.

Use at a dilution of: Coating: 1:100 - 1:500 Primary: 1:1000 - 1:30,000.

ICC/IF Use at an assay dependent concentration.
ICC 1/200 - 1/2000.
IHC - Wholemount 1/400.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

References for Anti-V5 tag antibody (ab9137)

This product has been referenced in:
  • Wong CC  et al. High-efficiency stem cell fusion-mediated assay reveals Sall4 as an enhancer of reprogramming. PLoS ONE 3:e1955 (2008). WB . Read more (PubMed: 18414659) »
  • Grskovic M  et al. Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells. PLoS Genet 3:e145 (2007). ChIP . Read more (PubMed: 17784790) »

See all 3 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.01% Triton-x 100
Specification
Brain
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Terry Yin

Verified customer

Submitted Aug 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T cells transfected with a solluble V5-tagge)
Specification
HEK293T cells transfected with a solluble V5-tagge
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% TritonX-100 in PBS for 15 minutes
Blocking step
Image-iT® FX Signal Enhancer (LifeTechnologies) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Mr. David Sanchez Martin

Verified customer

Submitted Jan 11 2013

I am very pleased to hear you would like to accept our offer and test ab9137 in ICC/IF and ab81397 in ICC. Each code will give you the VALUE of the product off an order before the expiration date. To redeem this offer, please submit an Abreview for ab9...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (Hindbrain)
Specification
Hindbrain
Username

Ms. Sana Zakaria

Verified customer

Submitted Oct 02 2012

Thank you for your reply.
I am pleased to hear you would like to participate in the testing discount scheme. The scheme is as you say, regardless of whether the result is a negative or a positive, you would still be entitled to free primary antibo...

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Thank you for contacting us.
Unfortunately, as you have discovered from the datasheet of the anti-V5 antibody (ab9137), this antibody has not been used in whole mount IHC to our knowledge as yet. This does not mean that the antibody would not work...

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The V5 epitope tag is used in transfection procedures in which the gene of interest is cloned in frame with the codons for the V5 epitope (GKPIPNPLLGLDST). The resulting expressed protein is tagged with the V5 epitope, which can be tracked with antibo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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