Overview

  • Product name
    Anti-V5 tag antibody (Agarose)
    See all V5 tag primary antibodies
  • Description
    Goat polyclonal to V5 tag (Agarose)
  • Conjugation
    Agarose
  • Tested applications
    Suitable for: IPmore details
  • Immunogen

    Full length native

    GKPIPNPLLGLDST

    (V5) (purified): conjugated to KLH.

  • General notes
    Provided as 400µl of slurry (200µl gel + 200µl buffer). 100µg of the affinity purified antibody is attached to the gel at a ratio of 500µg antibody/ml of gel or 250µg IgG/ml of 50% slurry.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Properties

Applications

Our Abpromise guarantee covers the use of ab1229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

References

This product has been referenced in:
  • Arumughan A  et al. Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers. Nat Commun 7:13047 (2016). IP ; Human . Read more (PubMed: 27762274) »
  • Garrett AM  et al. Replacing the PDZ-interacting C-termini of DSCAM and DSCAML1 with epitope tags causes different phenotypic severity in different cell populations. Elife 5:N/A (2016). IP . Read more (PubMed: 27637097) »

See all 9 Publications for this product

Customer reviews and Q&As

J’ai contacté le laboratoire et un tampon de RIPA pourrait peut-être fonctionner mais nous ne pouvons pas le confirmer car nous ne l’avons pas testé.

J’aimerais cependant mentionner qu’en utilisant u...

Read More
Application
Immunoprecipitation
Immuno-precipitation step
Other - ab1229
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Treatment
transfected with plasmid DNA
Total protein in input
5e+006 cells
Username

Ms. Christina Engel

Verified customer

Submitted Oct 10 2013



Our labs have tested this product using 0.1 % triton X-100.

Thank you for contacting us.
Please find below the purification procedure using antibodies on agarose beads:
1. The agarose beads with the antibody bound to them are ready for use when you receive them.
2. Place the beads in a column and...

Read More

Thank you for your enquiry. The buffer that we know it works in has a concentration of 0.1% SDS and 0.5% deoxycholate. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact...

Read More

Thank you for your enquiry. Theoretically this will work, but to the best of our knowledge it has not been proven to work satisfactorally. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. Following immunoprecipitation, some heavy and light IgG chains may be eluted with reducing buffers such as Laemmli. This may cause interference with your V5-tagged protein. The best and cleanest approach would be to IP u...

Read More

Thank you for your enquiry. Tris based buffers will not have an appreciable influence on the agarose immobilized antibody. Some heavy and light IgG chains will be eluted with reducing buffers such as Laemmli.

We acknowledge receipt and would like to thank you for your request of bulk quote for the ab1229. I am currently working on our best quotation and will be contacting you in the next couple of days.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up