GKPIPNPLLGLDST(V5 epitope) conjugated with KLH.
Our Abpromise guarantee covers the use of ab9116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 18575576|
|ChIP||Use at an assay dependent concentration. PubMed: 17289569|
|WB||Use at an assay dependent concentration. Can be blocked with V5 tag peptide (ab15829).|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ChIP/Chip||Use at an assay dependent concentration. PubMed: 17289569|
|ICC||Use at an assay dependent concentration. PubMed: 17012257|
This image is courtesy of an anonymous AbreviewMouse ES cells lysate.
ab9116 at 1/1000 dilution staining V5 in V5-Pgc transfected Drosophila Kc cells by Immunocytochemistry/ Immunofluorescence. Kc cells were fixed in 4% formaldehyde for 10 minutes, washed in PBS-Tween 20 (0.05%) and blocked in 5% milk in PBS-Tween 20 for 1 hour. Cells were incubated with primary antibody at 4°C overnight and the secondary antibody at room temperature for 3 hours.
ChIP qPCR: The graph on the left represents the input chromatin of PC3-EMT14-OVOL2 relative to empty vector (EV) control. The graph on the right depicts the ChIP DNA using V5 antibody (ab9116). The V5 epitope was included at the C-terminus of the expressed OVOL2. Results were normalized to input controls and graphs are relative to EV. Graphs show mean +/- sem.