Overview

  • Product nameAnti-V5 tag antibody - ChIP Grade
    See all V5 tag primary antibodies
  • Description
    Rabbit polyclonal to V5 tag - ChIP Grade
  • SpecificityELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
  • Tested applicationsSuitable for: ICC/IF, ChIP, WB, ELISA, IP, ChIP/Chip, ICCmore details
  • Immunogen

    GKPIPNPLLGLDST

    (V5 epitope) conjugated with KLH.

  • Positive control
    • Ab5395

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg .
  • ClonalityPolyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 18575576
ChIP Use at an assay dependent concentration. PubMed: 17289569
WB Use at an assay dependent concentration. Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ChIP/Chip Use at an assay dependent concentration. PubMed: 17289569
ICC Use at an assay dependent concentration. PubMed: 17012257

Target

  • RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody - ChIP Grade images

  • All lanes : Anti-V5 tag antibody - ChIP Grade (ab9116) at 1/5000 dilution

    Lane 1 : Cells transfected with v5-tagged protein.
    Lane 2 : Cells transfected with v5-tagged protein.
    Lane 3 : Cells transfected with v5-tagged protein.
    Lane 4 : Untransfected cells.
    Lane 5 : Untransfected cells.

    Secondary
    Lanes 1 - 3 : Anti-rabbit Ig-HRP at 1/20000 dilution
    Lanes 4 - 5 : Anti-rabbit Ig-HRP at 1/20000 dilution

    This image is courtesy of an anonymous Abreview

    Mouse ES cells lysate.

    See Abreview

  • ab9116 at 1/1000 dilution staining V5 in V5-Pgc transfected Drosophila Kc cells by Immunocytochemistry/ Immunofluorescence. Kc cells were fixed in 4% formaldehyde for 10 minutes, washed in PBS-Tween 20 (0.05%) and blocked in 5% milk in PBS-Tween 20 for 1 hour. Cells were incubated with primary antibody at 4°C overnight and the secondary antibody at room temperature for 3 hours.

  • ChIP qPCR: The graph on the left represents the input chromatin of PC3-EMT14-OVOL2 relative to empty vector (EV) control. The graph on the right depicts the ChIP DNA using V5 antibody (ab9116). The V5 epitope was included at the C-terminus of the expressed OVOL2. Results were normalized to input controls and graphs are relative to EV. Graphs show mean +/- sem.

References for Anti-V5 tag antibody - ChIP Grade (ab9116)

This product has been referenced in:

See all 51 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human u2os cells)
Permeabilization Yes - 0.1% triton
Specification human u2os cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 13 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH/3T3 fibroblasts with V5-tag constructs)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification NIH/3T3 fibroblasts with V5-tag constructs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Pawel Niewiadomski

Verified customer

Submitted Feb 09 2016

Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing
Loading amount 15 µg
Specification HEK293
Blocking step Thermo Scientific #EN37543 Starting Block TBST formulation as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Monkey Cell (cos7 cell)
Specification cos7 cell
Permeabilization Yes - 0.2% tritonx
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 19 2013


Nous n’avons pas testé cet anticorps dans nos laboratoires et donc nous n’avons pas de dilution spécifique à recommander pour ab9116 appart les informations dans la publication suivante :
Sugiyama T et al. SHREC, an effector complex for heter...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (293 cells transfected with a V5-tagged construct)
Loading amount 2000 cells
Specification 293 cells transfected with a V5-tagged construct
Gel Running Conditions Reduced Denaturing (10% AA gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 19 2012

Thank you for calling today, and I'm sorry to hear that our V5 antibody is giving you so much trouble!

As we discussed over the phone, I'm unsure of why the antibody is detecting that band in your samples and all of the protocol modifications ...

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I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. The code will give you the VALUE of each product off an order before the expiration date. To redeem this offer, please submit an Abreview for flow c...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your updates. I am very pleased to hear that you obtained good results.

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If you need any further assistance in the future, please do not hesitate to conta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"