Anti-V5 tag antibody [E10/V4RR] (HRP) (ab173837)

Overview

  • Product name
    Anti-V5 tag antibody [E10/V4RR] (HRP)
    See all V5 tag primary antibodies
  • Description
    Mouse monoclonal [E10/V4RR] to V5 tag (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide corresponding to V5 tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    GKPIPNPLLGLDST

  • Positive control
    • E. coli lysate containing a V5 multi-epitope tagged protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    E10/V4RR
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • All lanes : Anti-V5 tag antibody [E10/V4RR] (HRP) (ab173837) at 1/1000 dilution

    Lane 1 : E. coli lysate containing a V5 multi-epitope tagged protein at 1 µg
    Lane 2 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.5 µg
    Lane 3 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.25 µg
    Lane 4 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.125 µg
    Lane 5 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.0599999986589 µg
    Lane 6 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.0299999993294 µg

    Developed using the ECL technique

References

ab173837 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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