Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide immobilized on a solid phase and conjugated to fluorescein isothiocyanate.
  • ClonalityPolyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Can be blocked with V5 tag peptide (ab15829).
ELISA 1/5000 - 1/15000.
ICC/IF Use at an assay dependent concentration. PubMed: 21911501

Target

  • RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody (FITC) images

  • ab1209, staining V5 tag (green) in U373 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were transfected with TRIL-V5 and stimulated with 25 µg/ml PIC for 0 (left) and 4 hours (right). Samples were fixed with formaldehyde and permeabilized using 0.2% Triton X-100 for 5 minutes before being incubated with primary antibody.

References for Anti-V5 tag antibody (FITC) (ab1209)

This product has been referenced in:
  • Carpenter S  et al. Toll-like Receptor 3 (TLR3) Signaling Requires TLR4 Interactor with Leucine-rich Repeats (TRIL). J Biol Chem 286:38795-804 (2011). ICC/IF . Read more (PubMed: 21911501) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest in ab1209. To our knowledge, ab1209 has not been tested in IF. Therefore, I can offer a discount off a future purchase if you buy ab1209 now, test it inIF and submit feedback to us in the form of an Abreview. It do...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (neuroblastoma cells)
Specification neuroblastoma cells
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1 % Triton, 0.2 % BSA-PBS 1X
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Dr. Min Kang

Verified customer

Submitted Aug 15 2011

Thank you for your enquiry. This product has an expiration date of 12 months. If you need any further assistance please do not hesitate to get back in touch.

Good results have been gained with this antibody up to 200ng/ml for western blotting. As a detection antibody in ELISA assays it could be used up to 50ng/ml. For your purposes a starting concentration of 2ug/ml is reccomended.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"