• Product nameAnti-V5 tag antibody (HRP)
    See all V5 tag primary antibodies
  • Description
    Rabbit polyclonal to V5 tag (HRP)
  • ConjugationHRP
  • SpecificityIn a direct ELISA against 1 ug of V5 peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • Tested applicationsSuitable for: ICC, WB, ELISAmore details
  • Immunogen

    Synthetic peptide:


    conjugated to KLH.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid phase support and conjugated to horseradish peroxidase.
  • ClonalityPolyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab1325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent dilution.


  • RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

References for Anti-V5 tag antibody (HRP) (ab1325)

This product has been referenced in:
  • Gin P  et al. Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1. Hum Mol Genet 21:2961-72 (2012). ELISA . Read more (PubMed: 22493000) »
  • Levy D  et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). Read more (PubMed: 21131967) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 0.1 µg
Gel Running Conditions Reduced Denaturing (4~20%)
Sample Escherichia coli Recombinant protein (Recombinant protein with C-terminus V5 tag)
Specification Recombinant protein with C-terminus V5 tag
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 30 2014

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab15828. To check the status of the order please contact our Customer...

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Thank you for your enquiry. I am sorry to hear this vial of ab1325 is giving you trouble. It does appear that this antibody is not detecting the V5 tag as expected. If you have purchased it within the last 6 months or so I would be happy to offer you a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (Recombinant V5 tagged Protein)
Specification Recombinant V5 tagged Protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type Direct

Abcam user community

Verified customer

Submitted Aug 11 2008