PE Anti-V5 tag antibody (ab72480)
Key features and details
- PE Rabbit polyclonal to V5 tag
- Suitable for: Flow Cyt, Sandwich ELISA
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Overview
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Product name
PE Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
PE Rabbit polyclonal to V5 tag -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt, Sandwich ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide V5 Tag - Tagged
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General notes
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose -
Concentration information loading...
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Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab37409 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. ab37409 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Sandwich ELISA
Use at an assay dependent concentration. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72480 (red). Black - Mouse IgG1 Phycoerythrin isotype control.
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Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5 tagged peptide, and read after a 2 hour incubation at room temperature with ab72480.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab72480 has been referenced in 2 publications.
- Renner TM et al. Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus. J Virol 94:N/A (2020). PubMed: 31694951
- Dam Van P et al. Anti-Influenza A Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species. J Interferon Cytokine Res 39:274-282 (2019). PubMed: 30939061