The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab37409-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
GKPIPNPLLGLDST epitope tag antibody
GKPIPNPLLGLDST tag antibody
Protein Rev antibody
Regulator of expression of viral proteins antibody
V5 epitope tag antibody
Flow Cytometry - Anti-V5 tag antibody (Phycoerythrin) (ab72480)
Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72480 (red). Black - Mouse IgG1 Phycoerythrin isotype control.
Sandwich ELISA - Anti-V5 tag antibody (Phycoerythrin) (ab72480)
Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5 tagged peptide, and read after a 2 hour incubation at room temperature with ab72480.