Anti-V5 tag antibody (Phycoerythrin) (ab72480)

Overview

  • Product name
    Anti-V5 tag antibody (Phycoerythrin)
    See all V5 tag primary antibodies
  • Description
    Rabbit polyclonal to V5 tag (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Synthetic peptide V5 Tag - Tagged

  • General notes

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Purification notes
    Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab37409-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72480  (red). Black - Mouse IgG1 Phycoerythrin isotype control.

  • Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5 tagged peptide, and read after a 2 hour incubation at room temperature with ab72480.

References

ab72480 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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