Overview

  • Product nameAnti-V5 tag antibody [SV5-Pk1]
    See all V5 tag primary antibodies
  • Description
    Mouse monoclonal [SV5-Pk1] to V5 tag
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details
  • Immunogen

    Mice were infected with the paramyxovirus SV5, Simian-Virus 5.

  • Epitopeab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
  • General notes

    ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody was purified from tissue culture supernatant by affinity chromatography.
  • Primary antibody notesAb27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
  • ClonalityMonoclonal
  • Clone numberSV5-Pk1
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21887337
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/1000 - 1/5000.
IP Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
RIA Use at an assay dependent concentration.

Target

  • RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody [SV5-Pk1] images

  • Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution + mouse cortex cell line lysate at 30 µg

    Secondary
    Non-Abcam antibody was used: Vector Conjugation: HRP at 1/3000 dilution

    Melina Reisenberg, CARD, KCL, London

    See Abreview

  • ab27671 at a 1/1000 dilution staining V5 tag in CHO cells overexpressing a V5-tagged protein by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked with 3% BSA. The secondary used was an Alexa Fluor® 488 conjugated goat anti-mouse IgG (H+L) at a 1/1000 dilution.

References for Anti-V5 tag antibody [SV5-Pk1] (ab27671)

This product has been referenced in:

See all 24 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH3T3 cells)
Gel Running Conditions Reduced Denaturing (4%-12%)
Loading amount 20 µg
Treatment Transfected with or without V5-tagged plasmids
Specification NIH3T3 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (myoblast)
Gel Running Conditions Non-reduced Denaturing (10%)
Loading amount 40 µg
Treatment infected with retrovirus
Specification myoblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Miss. Can Zhou

Verified customer

Submitted Feb 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification vascular smooth muscle cell
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jan 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (HEK293)
Specification HEK293
Permeabilization Yes - 0.2% triton
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 19 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your kinds words. I am happy to help!

As requested, I have issued a free of charge replacement for one vial of ab27671.

To check the status of the order please contact our Customer Service team and reference this numbe...

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Thanks for your reply. I am sorry to hear the replacement vial of ab3366 did not improve things.

Just to make sure, you would like me to send along one vial of ab27671 as a replacement for this most recent faulty vial of ab3366? If this is yo...

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For ab27671 we only have data on it being used in HEK cells and for ab9116, it was used in HeLa and the western blot data is available on the public website, in the Abreview section. Both antibodies appeared to give a strong signal in cells transfected...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Cortex cell line)
Loading amount 30 µg
Specification Cortex cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Chinese Hamster Cultured Cells (CHO cells overexpressing a V5-tagged protein)
Specification CHO cells overexpressing a V5-tagged protein
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"