Overview

  • Product name
    Anti-V5 tag antibody [SV5-Pk1]
    See all V5 tag primary antibodies
  • Description
    Mouse monoclonal [SV5-Pk1] to V5 tag
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details
  • Immunogen

    Mice were infected with the paramyxovirus SV5, Simian-Virus 5.

  • Epitope
    ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
  • General notes

    ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified from tissue culture supernatant by affinity chromatography.
  • Primary antibody notes
    Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
  • Clonality
    Monoclonal
  • Clone number
    SV5-Pk1
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21887337
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/1000 - 1/5000.
IP Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
RIA Use at an assay dependent concentration.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • ab27671 at a 1/1000 dilution staining V5 tag in CHO cells overexpressing a V5-tagged protein by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked with 3% BSA. The secondary used was an Alexa Fluor® 488 conjugated goat anti-mouse IgG (H+L) at a 1/1000 dilution.
  • Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution + mouse cortex cell line lysate at 30 µg

    Secondary
    Non-Abcam antibody was used: Vector Conjugation: HRP at 1/3000 dilution

    This image is courtesy of an abreview submitted by Melina Reisenberg, CARD, KCL, London

    See Abreview

References

This product has been referenced in:
  • Kim D  et al. Homo-trimerization is essential for the transcription factor function of Myrf for oligodendrocyte differentiation. Nucleic Acids Res 45:5112-5125 (2017). Read more (PubMed: 28160598) »
  • Moleirinho S  et al. Regulation of localization and function of the transcriptional co-activator YAP by angiomotin. Elife 6:N/A (2017). WB, IP ; Human . Read more (PubMed: 28464980) »

See all 42 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3 cells)
Gel Running Conditions
Reduced Denaturing (4%-12%)
Loading amount
20 µg
Treatment
Transfected with or without V5-tagged plasmids
Specification
NIH3T3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (myoblast)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
40 µg
Treatment
infected with retrovirus
Specification
myoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Miss. Can Zhou

Verified customer

Submitted Feb 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
vascular smooth muscle cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jan 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Human Cell (HEK293)
Specification
HEK293
Permeabilization
Yes - 0.2% triton
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cortex cell line)
Loading amount
30 µg
Specification
Cortex cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Chinese Hamster Cultured Cells (CHO cells overexpressing a V5-tagged protein)
Specification
CHO cells overexpressing a V5-tagged protein
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
5 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Transfected S2 Cells expressing V5 Tagged protein)
Loading amount
20 µg
Specification
Transfected S2 Cells expressing V5 Tagged protein
Treatment
1mM CuSO4 for 24hrs
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Craig Faulkner

Verified customer

Submitted May 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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