Overview

  • Product name
    Anti-V5 tag antibody [SV5-Pk1] (FITC)
    See all V5 tag primary antibodies
  • Description
    Mouse monoclonal [SV5-Pk1] to V5 tag (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Immunogen

    Mice were infected with the paramyxovirus SV5, Simian-Virus 5

  • Epitope
    Ab27674 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
  • General notes


    Ab27674 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide.
    Constituents: 1% BSA.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Ab27674 was purified from tissue culture supernatant by affinity chromatography.
  • Primary antibody notes
    Ab27674 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
  • Clonality
    Monoclonal
  • Clone number
    SV5-Pk1
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

References for Anti-V5 tag antibody [SV5-Pk1] (FITC) (ab27674)

This product has been referenced in:
  • Sabirzhanov B  et al. Downregulation of miR-23a and miR-27a following experimental traumatic brain injury induces neuronal cell death through activation of proapoptotic Bcl-2 proteins. J Neurosci 34:10055-71 (2014). Read more (PubMed: 25057207) »
  • Sabirzhanov B  et al. Over-expression of HSP70 attenuates caspase-dependent and caspase-independent pathways and inhibits neuronal apoptosis. J Neurochem 123:542-54 (2012). Read more (PubMed: 22909049) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. I can confirm that this product is indeed a FITC conjugated antibody and will not require any secondary antibodies. The list of recommended secondaries you see in our datasheet is an error and we have been informed of it...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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