V5 tag peptide (ab15829)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
Description
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Product name
V5 tag peptide -
Purity
> 90 % HPLC. -
Accession
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Animal free
No -
Nature
Synthetic -
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Species
Parainfluenza virus 5
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Associated products
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Corresponding Antibody
Specifications
Our Abpromise guarantee covers the use of ab15829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
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Form
Lyophilized -
Additional notes
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
Information available upon request.
General Info
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Alternative names
- GKPIPNPLLGLDST epitope tag
- GKPIPNPLLGLDST tag
- Protein Rev
see all -
Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Images
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Lane 1: Primary: ab15828, 0.5 ug/ml
Sample: Recombinant V5 tagged Groucho homolog, 20 ug
Secondary: Goat anti-Rabbit IgG, 1/10000 dilutionLane 2: Primary: ab15828, 0.5 ug/ml
Blocking peptide: ab15829, 1 ug/ml
Sample: Recombinant V5 tagged Groucho , 20 ug
Secondary: Goat anti-Rabbit IgG, 1/10000 dilution
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab15829 has been referenced in 2 publications.
- Emery A et al. Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library. Cell Chem Biol 28:1602-1615.e9 (2021). PubMed: 34111400
- Dou K et al. Drosophila P75 safeguards oogenesis by preventing H3K9me2 spreading. J Genet Genomics 47:187-199 (2020). PubMed: 32499180